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anti nephrin antibody  (R&D Systems)


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    R&D Systems anti nephrin antibody
    Anti Nephrin Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nephrin/Human+Nephrin+Antibody/pmc12926970-35-27-33
    Average 95 stars, based on 109 article reviews
    anti nephrin antibody - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Mouse Assay:

    Article Title: MAGI-2 Regulates the Organization of Podocyte Actin Cytoskeleton Through its Interaction with α-Actinin-4 and Synaptopodin
    Article Snippet: .. Published by Wolters Kluwer Health, Inc. on behalf of the American Society of Nephrology Mice kidney sections were labeled with anti-MAGI-2 (M2441, rabbit, 1:100; Sigma-Aldrich), anti-synaptopodin (65194, mouse, 1:10; PROGEN), anti-α-actinin-4 (0042-05, rabbit, 1:200; immunoGrobe), Rhodamine Phalloidin (R415, Invitrogen), Podocalyxin (AF1556-SP, goat, 1:70; R&D Systems), Nidogen (ab14511, rabbit, 1:200; abcam), Nephrin (AF3159, goat, 1:100; R&D Systems), and ZO-1(sc33725, mouse, 1:50; Santa Cruz Biotechnology). ..

    Article Title: Membrane-Associated Guanylate Kinase Inverted 2 Regulates the Organization of Podocyte Actin Cytoskeleton through Its Interaction with α -Actinin-4 and Synaptopodin
    Article Snippet: IF images were obtained using a Zeiss LSM780 (Zeiss, Oberkochen, Germany) and Nikon structured illumination microscopy (Nikon Instruments Inc., Tokyo, Japan). .. Mice kidney sections were labeled with anti-MAGI-2 (M2441, rabbit, 1:100; Sigma-Aldrich), anti-Synpo (65,194, mouse, 1:10; PROGEN), anti- α -actinin-4 (0042-05, rabbit, 1:200; immunoGrobe), Rhodamine Phalloidin (R415, Invitrogen), Podocalyxin (AF1556-SP, goat, 1:70; R&D Systems), Nidogen (ab14511, rabbit, 1:200; abcam), Nephrin (AF3159, goat, 1:100; R&D Systems), and zonula occludens-1 (ZO-1) (sc33725, mouse, 1:50; Santa Cruz Biotechnology). ..

    Labeling:

    Article Title: MAGI-2 Regulates the Organization of Podocyte Actin Cytoskeleton Through its Interaction with α-Actinin-4 and Synaptopodin
    Article Snippet: .. Published by Wolters Kluwer Health, Inc. on behalf of the American Society of Nephrology Mice kidney sections were labeled with anti-MAGI-2 (M2441, rabbit, 1:100; Sigma-Aldrich), anti-synaptopodin (65194, mouse, 1:10; PROGEN), anti-α-actinin-4 (0042-05, rabbit, 1:200; immunoGrobe), Rhodamine Phalloidin (R415, Invitrogen), Podocalyxin (AF1556-SP, goat, 1:70; R&D Systems), Nidogen (ab14511, rabbit, 1:200; abcam), Nephrin (AF3159, goat, 1:100; R&D Systems), and ZO-1(sc33725, mouse, 1:50; Santa Cruz Biotechnology). ..

    Article Title: Membrane-Associated Guanylate Kinase Inverted 2 Regulates the Organization of Podocyte Actin Cytoskeleton through Its Interaction with α -Actinin-4 and Synaptopodin
    Article Snippet: IF images were obtained using a Zeiss LSM780 (Zeiss, Oberkochen, Germany) and Nikon structured illumination microscopy (Nikon Instruments Inc., Tokyo, Japan). .. Mice kidney sections were labeled with anti-MAGI-2 (M2441, rabbit, 1:100; Sigma-Aldrich), anti-Synpo (65,194, mouse, 1:10; PROGEN), anti- α -actinin-4 (0042-05, rabbit, 1:200; immunoGrobe), Rhodamine Phalloidin (R415, Invitrogen), Podocalyxin (AF1556-SP, goat, 1:70; R&D Systems), Nidogen (ab14511, rabbit, 1:200; abcam), Nephrin (AF3159, goat, 1:100; R&D Systems), and zonula occludens-1 (ZO-1) (sc33725, mouse, 1:50; Santa Cruz Biotechnology). ..

    Recombinant:

    Article Title: Development and provisional validation of a multiplex LC-MRM-MS test for timely kidney injury detection in urine
    Article Snippet: Recombinant SLC22A2 (Cat. Nr. H00006582-P01) was from Abnova (Taipei, Taiwan). .. Recombinant nephrin (Cat. Nr. 9399-NN-050) and biotinylated polyclonal antibodies against human TIMP2 (Cat. Nr. BAF971), IGFBP7 (Cat. Nr. BAF1334), KIM-1 (Cat. Nr. BAF1750), NGAL (Cat. Nr. BAF1757), CXCL9 (Cat. Nr. BAF392), cubilin (Cat. Nr. BAF3700), TGF-β1 (Cat. Nr. BAF246) and nephrin (Cat. Nr. BAF4269), were purchased from R&D systems (Minneapolis, Mn, USA). .. Recombinant KIM-1 (Cat. Nr. LS-G97633) was from LS Biologicals (Seattle, WA, USA) and recombinant CXCL9 (Cat. Nr. ab151917) and 3 TGF-β1 (Cat. Nr. ab50038) were from Abcam (Cambridge, UK).

    other:

    Article Title: Evolving adeno-associated viruses for gene transfer to the kidney via cross-species cycling of capsid libraries.
    Article Snippet: Secondary Antibodies: donkey anti-rabbit 488 (Invitrogen A21206), donkey anti-sheep 594 (Invitrogen A11016), donkey anti-rat 647 (Invitrogen A48272), goat anti-rabbit 594 (Invitrogen A11012), LTL-FL (Vector Laboratories FL-1321-2), donkey anti-rat 594 (A21209), donkey anti-mouse 647 (Invitrogen A31571), donkey anti-goat 488 (Invitrogen A-11055), DBA-FL (Vector Laboratories FL-1031), goat anti-rabbit Ig, Human ads-HRP (1:5000; Southern Biotech #4010).

    Chromatin Immunoprecipitation:

    Article Title: Evolving adeno-associated viruses for gene transfer to the kidney via cross-species cycling of capsid libraries.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies: anti-mCherry (Novus Biologics NBP2-25158), Nephrin (R&D Systems AF4269-SP), mCherry (Invitrogen M11217), anti-RFP (Rockland 600-401-379), SGLT1 (Izumi Kaji Lab 576-610), SGLT2 (Santa Cruz sc-393350), mouse nephrin (R&D Systems AF3159), vinculin (E1E9V) XP (1:5000; Cell Signaling #13901), anti-HA-Tag (1:1000; Invitrogen #PA1-985). .. Secondary Antibodies: donkey anti-rabbit 488 (Invitrogen A21206), donkey anti-sheep 594 (Invitrogen A11016), donkey anti-rat 647 (Invitrogen A48272), goat anti-rabbit 594 (Invitrogen A11012), LTL-FL (Vector Laboratories FL-1321-2), donkey anti-rat 594 (A21209), donkey anti-mouse 647 (Invitrogen A31571), donkey anti-goat 488 (Invitrogen A-11055), DBA-FL (Vector Laboratories FL-1031), goat anti-rabbit Ig, Human ads-HRP (1:5000; Southern Biotech #4010).

    Article Title: Engineering kidney developmental trajectory using culture boundary conditions
    Article Snippet: .. There were no analyses where decision-making by the researchers would have been required or could have impacted the findings. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) EPCAM-FITC, Invitrogen, Cat#11-5791-82, AB_11151709, clone G8.8; ECAD-Alexa 488, Invitrogen, Cat#53-3249-82, AB_10671003, clone DECMA-1; JAG1, R&D systems, AF599, AB_2128257; JAG1-PE, Invitrogen, 12-3391-82, AB_1963616, clone HMJ1-29; SIX2, Proteintech, 66347-1-Ig, AB_2881727, clone No.1F9G11; SIX2, Proteintech, 11562-1-AP, AB_2189084; ECAD, Invitrogen, 14-3249-82, AB_1210458, clone DECMA-1; RET, Cell signaling technology, 3223S, AB_2238465, clone C31B4; PDGFRa, R&D systems, AF1062, AB_2236897; Fibronectin, Abcam, Ab23750, AB_447655; Laminin, Abcam, Ab11575, AB_298179; Collagen I, Sigma, MAB3391, AB_94839, clone 5D8-G9; Nephrin, R&D systems, AF4269, AB_2154851, clone G-8; PECAM-PE, Invitrogen, 12-0311-82, AB_465632, clone 390; Lotus tetragonolobus lectin (LTL) - Biotinylated, Vector Laboratories, B-1325-2; anti-Rabbit Alexa 647, ThermoFisher, A-31573, AB_2536183; anti-Rabbit Alexa 488, ThermoFisher, A-21206, AB_2535792; anti-Rabbit Alexa 405, ThermoFisher, A-31573, AB_2536183; anti-Goat Alexa 555, ThermoFisher, A-21432, AB_2535853; anti-Mouse Alexa 555, ThermoFisher, A-31572, AB_162543; anti-Rat Alexa 488, ThermoFisher, A-21208, AB_2535794; anti-Sheep Alexa 647, ThermoFisher, A-21448, AB_2535865; DyLightTM 405 Streptavidin, Jackson ImmunoResearch, 016-470-084 The antibodies are commercially available and verified by their manufacturers. ..

    Flow Cytometry:

    Article Title: Evolving adeno-associated viruses for gene transfer to the kidney via cross-species cycling of capsid libraries.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies: anti-mCherry (Novus Biologics NBP2-25158), Nephrin (R&D Systems AF4269-SP), mCherry (Invitrogen M11217), anti-RFP (Rockland 600-401-379), SGLT1 (Izumi Kaji Lab 576-610), SGLT2 (Santa Cruz sc-393350), mouse nephrin (R&D Systems AF3159), vinculin (E1E9V) XP (1:5000; Cell Signaling #13901), anti-HA-Tag (1:1000; Invitrogen #PA1-985). .. Secondary Antibodies: donkey anti-rabbit 488 (Invitrogen A21206), donkey anti-sheep 594 (Invitrogen A11016), donkey anti-rat 647 (Invitrogen A48272), goat anti-rabbit 594 (Invitrogen A11012), LTL-FL (Vector Laboratories FL-1321-2), donkey anti-rat 594 (A21209), donkey anti-mouse 647 (Invitrogen A31571), donkey anti-goat 488 (Invitrogen A-11055), DBA-FL (Vector Laboratories FL-1031), goat anti-rabbit Ig, Human ads-HRP (1:5000; Southern Biotech #4010).

    Article Title: Engineering kidney developmental trajectory using culture boundary conditions
    Article Snippet: .. There were no analyses where decision-making by the researchers would have been required or could have impacted the findings. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) EPCAM-FITC, Invitrogen, Cat#11-5791-82, AB_11151709, clone G8.8; ECAD-Alexa 488, Invitrogen, Cat#53-3249-82, AB_10671003, clone DECMA-1; JAG1, R&D systems, AF599, AB_2128257; JAG1-PE, Invitrogen, 12-3391-82, AB_1963616, clone HMJ1-29; SIX2, Proteintech, 66347-1-Ig, AB_2881727, clone No.1F9G11; SIX2, Proteintech, 11562-1-AP, AB_2189084; ECAD, Invitrogen, 14-3249-82, AB_1210458, clone DECMA-1; RET, Cell signaling technology, 3223S, AB_2238465, clone C31B4; PDGFRa, R&D systems, AF1062, AB_2236897; Fibronectin, Abcam, Ab23750, AB_447655; Laminin, Abcam, Ab11575, AB_298179; Collagen I, Sigma, MAB3391, AB_94839, clone 5D8-G9; Nephrin, R&D systems, AF4269, AB_2154851, clone G-8; PECAM-PE, Invitrogen, 12-0311-82, AB_465632, clone 390; Lotus tetragonolobus lectin (LTL) - Biotinylated, Vector Laboratories, B-1325-2; anti-Rabbit Alexa 647, ThermoFisher, A-31573, AB_2536183; anti-Rabbit Alexa 488, ThermoFisher, A-21206, AB_2535792; anti-Rabbit Alexa 405, ThermoFisher, A-31573, AB_2536183; anti-Goat Alexa 555, ThermoFisher, A-21432, AB_2535853; anti-Mouse Alexa 555, ThermoFisher, A-31572, AB_162543; anti-Rat Alexa 488, ThermoFisher, A-21208, AB_2535794; anti-Sheep Alexa 647, ThermoFisher, A-21448, AB_2535865; DyLightTM 405 Streptavidin, Jackson ImmunoResearch, 016-470-084 The antibodies are commercially available and verified by their manufacturers. ..

    Magnetic Resonance Imaging:

    Article Title: Evolving adeno-associated viruses for gene transfer to the kidney via cross-species cycling of capsid libraries.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies: anti-mCherry (Novus Biologics NBP2-25158), Nephrin (R&D Systems AF4269-SP), mCherry (Invitrogen M11217), anti-RFP (Rockland 600-401-379), SGLT1 (Izumi Kaji Lab 576-610), SGLT2 (Santa Cruz sc-393350), mouse nephrin (R&D Systems AF3159), vinculin (E1E9V) XP (1:5000; Cell Signaling #13901), anti-HA-Tag (1:1000; Invitrogen #PA1-985). .. Secondary Antibodies: donkey anti-rabbit 488 (Invitrogen A21206), donkey anti-sheep 594 (Invitrogen A11016), donkey anti-rat 647 (Invitrogen A48272), goat anti-rabbit 594 (Invitrogen A11012), LTL-FL (Vector Laboratories FL-1321-2), donkey anti-rat 594 (A21209), donkey anti-mouse 647 (Invitrogen A31571), donkey anti-goat 488 (Invitrogen A-11055), DBA-FL (Vector Laboratories FL-1031), goat anti-rabbit Ig, Human ads-HRP (1:5000; Southern Biotech #4010).

    Article Title: Engineering kidney developmental trajectory using culture boundary conditions
    Article Snippet: .. There were no analyses where decision-making by the researchers would have been required or could have impacted the findings. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) EPCAM-FITC, Invitrogen, Cat#11-5791-82, AB_11151709, clone G8.8; ECAD-Alexa 488, Invitrogen, Cat#53-3249-82, AB_10671003, clone DECMA-1; JAG1, R&D systems, AF599, AB_2128257; JAG1-PE, Invitrogen, 12-3391-82, AB_1963616, clone HMJ1-29; SIX2, Proteintech, 66347-1-Ig, AB_2881727, clone No.1F9G11; SIX2, Proteintech, 11562-1-AP, AB_2189084; ECAD, Invitrogen, 14-3249-82, AB_1210458, clone DECMA-1; RET, Cell signaling technology, 3223S, AB_2238465, clone C31B4; PDGFRa, R&D systems, AF1062, AB_2236897; Fibronectin, Abcam, Ab23750, AB_447655; Laminin, Abcam, Ab11575, AB_298179; Collagen I, Sigma, MAB3391, AB_94839, clone 5D8-G9; Nephrin, R&D systems, AF4269, AB_2154851, clone G-8; PECAM-PE, Invitrogen, 12-0311-82, AB_465632, clone 390; Lotus tetragonolobus lectin (LTL) - Biotinylated, Vector Laboratories, B-1325-2; anti-Rabbit Alexa 647, ThermoFisher, A-31573, AB_2536183; anti-Rabbit Alexa 488, ThermoFisher, A-21206, AB_2535792; anti-Rabbit Alexa 405, ThermoFisher, A-31573, AB_2536183; anti-Goat Alexa 555, ThermoFisher, A-21432, AB_2535853; anti-Mouse Alexa 555, ThermoFisher, A-31572, AB_162543; anti-Rat Alexa 488, ThermoFisher, A-21208, AB_2535794; anti-Sheep Alexa 647, ThermoFisher, A-21448, AB_2535865; DyLightTM 405 Streptavidin, Jackson ImmunoResearch, 016-470-084 The antibodies are commercially available and verified by their manufacturers. ..

    Biomarker Discovery:

    Article Title: Engineering kidney developmental trajectory using culture boundary conditions
    Article Snippet: .. There were no analyses where decision-making by the researchers would have been required or could have impacted the findings. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) EPCAM-FITC, Invitrogen, Cat#11-5791-82, AB_11151709, clone G8.8; ECAD-Alexa 488, Invitrogen, Cat#53-3249-82, AB_10671003, clone DECMA-1; JAG1, R&D systems, AF599, AB_2128257; JAG1-PE, Invitrogen, 12-3391-82, AB_1963616, clone HMJ1-29; SIX2, Proteintech, 66347-1-Ig, AB_2881727, clone No.1F9G11; SIX2, Proteintech, 11562-1-AP, AB_2189084; ECAD, Invitrogen, 14-3249-82, AB_1210458, clone DECMA-1; RET, Cell signaling technology, 3223S, AB_2238465, clone C31B4; PDGFRa, R&D systems, AF1062, AB_2236897; Fibronectin, Abcam, Ab23750, AB_447655; Laminin, Abcam, Ab11575, AB_298179; Collagen I, Sigma, MAB3391, AB_94839, clone 5D8-G9; Nephrin, R&D systems, AF4269, AB_2154851, clone G-8; PECAM-PE, Invitrogen, 12-0311-82, AB_465632, clone 390; Lotus tetragonolobus lectin (LTL) - Biotinylated, Vector Laboratories, B-1325-2; anti-Rabbit Alexa 647, ThermoFisher, A-31573, AB_2536183; anti-Rabbit Alexa 488, ThermoFisher, A-21206, AB_2535792; anti-Rabbit Alexa 405, ThermoFisher, A-31573, AB_2536183; anti-Goat Alexa 555, ThermoFisher, A-21432, AB_2535853; anti-Mouse Alexa 555, ThermoFisher, A-31572, AB_162543; anti-Rat Alexa 488, ThermoFisher, A-21208, AB_2535794; anti-Sheep Alexa 647, ThermoFisher, A-21448, AB_2535865; DyLightTM 405 Streptavidin, Jackson ImmunoResearch, 016-470-084 The antibodies are commercially available and verified by their manufacturers. ..



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    Image Search Results


    Assay to detect nephrin autoantibodies.

    Journal: Annals of Medicine

    Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

    doi: 10.1080/07853890.2026.2661115

    Figure Lengend Snippet: Assay to detect nephrin autoantibodies.

    Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

    Techniques:

    Detection of serum anti-nephrin autoantibodies by ELISA. (a) Serum levels of anti-nephrin autoantibodies in patients with minimal change disease (MCD), primary focal segmental glomerulosclerosis (pFSGS), membranous nephropathy (MN), lupus nephritis (LN), diabetic nephropathy (DN), IgA nephropathy (IgAN), and healthy controls. (b) Positive rates of anti-nephrin autoantibodies in the same cohorts.

    Journal: Annals of Medicine

    Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

    doi: 10.1080/07853890.2026.2661115

    Figure Lengend Snippet: Detection of serum anti-nephrin autoantibodies by ELISA. (a) Serum levels of anti-nephrin autoantibodies in patients with minimal change disease (MCD), primary focal segmental glomerulosclerosis (pFSGS), membranous nephropathy (MN), lupus nephritis (LN), diabetic nephropathy (DN), IgA nephropathy (IgAN), and healthy controls. (b) Positive rates of anti-nephrin autoantibodies in the same cohorts.

    Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

    Techniques: Enzyme-linked Immunosorbent Assay

    Serum anti-nephrin autoantibody profiles across disease activity states in patients with MCD. (a) Serum levels of anti-nephrin autoantibodies in patients during active phase, partial remission (PR), and complete remission (CR). (b) Positivity rates of anti-nephrin autoantibodies across disease activity states. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Annals of Medicine

    Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

    doi: 10.1080/07853890.2026.2661115

    Figure Lengend Snippet: Serum anti-nephrin autoantibody profiles across disease activity states in patients with MCD. (a) Serum levels of anti-nephrin autoantibodies in patients during active phase, partial remission (PR), and complete remission (CR). (b) Positivity rates of anti-nephrin autoantibodies across disease activity states. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

    Techniques: Activity Assay

    Correlations between serum anti-nephrin autoantibody levels and key clinical parameters.

    Journal: Annals of Medicine

    Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

    doi: 10.1080/07853890.2026.2661115

    Figure Lengend Snippet: Correlations between serum anti-nephrin autoantibody levels and key clinical parameters.

    Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

    Techniques:

    Comparison of clinical characteristics between patients positive and negative for anti-nephrin autoantibodies. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Annals of Medicine

    Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

    doi: 10.1080/07853890.2026.2661115

    Figure Lengend Snippet: Comparison of clinical characteristics between patients positive and negative for anti-nephrin autoantibodies. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

    Techniques: Comparison

    Anti-nephrin autoantibody IgG subclasses in patients with MCD. (a) Proportion (%) of IgG subclasses among anti-nephrin autoantibodies. (b) Correlation of anti‑nephrin-IgG4 with total anti‑nephrin-IgG. (c) Ratios of anti-nephrin-IgG subclasses indicative of Th1/Th2 skewing: IgG1/IgG4 and IgG3/IgG4.

    Journal: Annals of Medicine

    Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

    doi: 10.1080/07853890.2026.2661115

    Figure Lengend Snippet: Anti-nephrin autoantibody IgG subclasses in patients with MCD. (a) Proportion (%) of IgG subclasses among anti-nephrin autoantibodies. (b) Correlation of anti‑nephrin-IgG4 with total anti‑nephrin-IgG. (c) Ratios of anti-nephrin-IgG subclasses indicative of Th1/Th2 skewing: IgG1/IgG4 and IgG3/IgG4.

    Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

    Techniques:

    (a) OCT frozen kidney biopsies were sectioned, cleared and immunolabelled followed by confocal imaging and deep-learning segmentation. (b) Maximum intensity projection confocal image of nephrin visualizing the slit diaphragm network and foot processes. (c) Output of the semantic segmentation of both the slit diaphragm and foot processes. Foot processes are marked in red and the slit diaphragm in green. (d) Output of the instance segmentation resulting in segmentation of each individual foot process in the image. Each foot process is presented in an individual color. (e-g) From the 2 segmentation outputs, the morphometric parameters SDL (e), FP circularity (f), FP area and FP perimeter (g) are extracted. (e) Schematic image of how SDL is measured. The length of the slit (in blue) is divided by the surface area (outlined in white) resulting in a length-density measurement of slit coverage. (f) Schematic image of how FP circularity is measured. From the segmentation of the individual foot processes, the FP circularity is calculated using the formula, circularity = 4 π (area/perimeter−2). The result is a value between 0 and 1 where a perfect circle has a circularity of 1 and a very elongated rectangle approaches 0. (g) Foot process size is calculated both by FP area and by FP perimeter. The FP area is the pixel area of each segmented foot process converted to µm 2 and the FP perimeter is the outline of the foot process with it closed at the base.

    Journal: medRxiv

    Article Title: Nanoscale Podocyte Morphometrics Predict Disease Progression in IgA Nephropathy

    doi: 10.64898/2026.03.30.26349728

    Figure Lengend Snippet: (a) OCT frozen kidney biopsies were sectioned, cleared and immunolabelled followed by confocal imaging and deep-learning segmentation. (b) Maximum intensity projection confocal image of nephrin visualizing the slit diaphragm network and foot processes. (c) Output of the semantic segmentation of both the slit diaphragm and foot processes. Foot processes are marked in red and the slit diaphragm in green. (d) Output of the instance segmentation resulting in segmentation of each individual foot process in the image. Each foot process is presented in an individual color. (e-g) From the 2 segmentation outputs, the morphometric parameters SDL (e), FP circularity (f), FP area and FP perimeter (g) are extracted. (e) Schematic image of how SDL is measured. The length of the slit (in blue) is divided by the surface area (outlined in white) resulting in a length-density measurement of slit coverage. (f) Schematic image of how FP circularity is measured. From the segmentation of the individual foot processes, the FP circularity is calculated using the formula, circularity = 4 π (area/perimeter−2). The result is a value between 0 and 1 where a perfect circle has a circularity of 1 and a very elongated rectangle approaches 0. (g) Foot process size is calculated both by FP area and by FP perimeter. The FP area is the pixel area of each segmented foot process converted to µm 2 and the FP perimeter is the outline of the foot process with it closed at the base.

    Article Snippet: OCT-frozen biopsy specimens were fixed in PFA, cleared in 4% SDS w/ boric acid and fluorescently immunolabelled for nephrin (R&D Systems, cat. no. AF4269) to visualize the slit diaphragm and podocyte foot processes (FPs) based on the protocol described by Unnersjö-Jess et al (details available in Supplement Methods).

    Techniques: Imaging